abstract：:Bionanocapsules (BNCs) are hollow nanoscale particles composed of L protein of the hepatitis B virus surface antigen that represent specific affinity for human hepatocytes. BNCs can transfer genes and drugs into human hepatocytes efficiently and specifically. BNC can be expressed in yeast cells. In this study, we deve...

journal_title：Applied microbiology and biotechnology

authors： Shishido T,Muraoka M,Ueda M,Seno M,Tanizawa K,Kuroda S,Fukuda H,Kondo A

更新日期：2006-12-01 00:00:00

abstract：:A 6-kDa antifungal peptide with inhibitory activity on mycelial growth in Fusarium oxysporum, Mycosphaerella arachidicola, and Physalospora piricola was isolated from baby lima beans. The peptide suppressed growth in M. arachidicola with an IC(50) of 0.87 muM and inhibited activity of HIV-1 reverse transcriptase with ...

journal_title：Applied microbiology and biotechnology

authors： Wang HX,Ng TB

更新日期：2006-12-01 00:00:00

abstract：:A chromosomal DNA fragment of 7.8 kb from Lactobacillus coryniformis CECT 5711 was cloned in Escherichia coli K-12 and was found to express a functional beta-galactosidase. Nucleotide sequence analysis showed that this fragment contained two partially overlapping genes, the lacL (1,881 bp) and the lacM (960 bp), that ...

journal_title：Applied microbiology and biotechnology

authors： Corral JM,Bañuelos O,Adrio JL,Velasco J

更新日期：2006-12-01 00:00:00

abstract：:In this study, the kinetic behaviors between n-butyl acetate and composite bead were investigated. Both microbial growth rate and biochemical reaction rate would be inhibited with increasing average inlet concentration. The order of the inhibitive effect, which resulted from increased average inlet concentration for f...

journal_title：Applied microbiology and biotechnology

authors： Chan WC,Hwang ID

更新日期：2006-11-01 00:00:00

abstract：:DNA microarrays have found widespread use as a flexible tool to investigate bacterial metabolism. Their main advantage is the comprehensive data they produce on the transcriptional response of the whole genome to an environmental or genetic stimulus. This allows the microbiologist to monitor metabolism and to define s...

journal_title：Applied microbiology and biotechnology

authors： Ehrenreich A

更新日期：2006-11-01 00:00:00

abstract：:Brazzein is an intensely sweet-tasting plant protein with good stability, which makes it an attractive alternative to sucrose. A brazzein gene has been designed, synthesized, and expressed in Escherichia coli at 30 degrees C to yield brazzein in a soluble form and in considerable quantity. Antibodies have been produce...

journal_title：Applied microbiology and biotechnology

authors： Berlec A,Jevnikar Z,Majhenic AC,Rogelj I,Strukelj B

更新日期：2006-11-01 00:00:00

abstract：:Using molecular techniques and sequencing, we studied the intraspecific diversity of Oenococcus oeni, a lactic acid bacterium involved in red winemaking. A relationship between the phenotypic and genotypic characterization of 16 O. oeni strains isolated from wine with different levels of enological potential was shown...

journal_title：Applied microbiology and biotechnology

authors： Delaherche A,Bon E,Dupé A,Lucas M,Arveiler B,De Daruvar A,Lonvaud-Funel A

更新日期：2006-11-01 00:00:00

abstract：:A simple, two-step efficient method to perform multiple-site mutagenesis of a gene from bacterial genome was developed. The method was named polyacrylamide gel electrophoresis (PAGE)-mediated overlap extension polymerase chain reaction (PCR) (POEP). The first step involves synthesis of individual fragments containing ...

journal_title：Applied microbiology and biotechnology

authors： Peng RH,Xiong AS,Yao QH

更新日期：2006-11-01 00:00:00

abstract：:The 5'-untranslated leader sequence (UTLS) of the slpA gene from Lactobacillus acidophilus contributes to mRNA stabilization by producing a 5' stem and loop structure, and a high-level expression system for the lactic acid bacteria was developed using the UTLS in this study. A plasmid, which expresses alpha-amylase un...

journal_title：Applied microbiology and biotechnology

authors： Narita J,Ishida S,Okano K,Kimura S,Fukuda H,Kondo A

更新日期：2006-11-01 00:00:00

abstract：:The production of mannitol by Lactobacillus intermedius NNRL B-3693 using molasses as an inexpensive carbon source was evaluated. The bacterium produced mannitol (104 g/l) from molasses and fructose syrups (1:1; total sugars, 150 g/l; fructose:glucose 4:1) in 16 h. Several kinds of inexpensive organic and inorganic ni...

journal_title：Applied microbiology and biotechnology

authors： Saha BC

更新日期：2006-10-01 00:00:00

abstract：:Lipophilic extractives in wood and other lignocellulosic materials exert a negative impact in pulp and paper manufacturing causing the so-called pitch problems. In this work, the appropriateness of an enzymatic treatment using the laccase-mediator system for pitch biocontrol is evaluated. With this purpose, three pulp...

journal_title：Applied microbiology and biotechnology

authors： Gutiérrez A,del Río JC,Rencoret J,Ibarra D,Martínez AT

更新日期：2006-10-01 00:00:00

abstract：:Streptococcus mutans is the main cause of dental caries. We evaluated the therapeutic effect of variable regions of a llama heavy chain antibody fragments directed against S. mutans named S36-VHH (S for Streptococcus) alone or fused with glucose oxidase (GOx) from Aspergillus niger. Western blot analysis and ELISA rev...

journal_title：Applied microbiology and biotechnology

authors： Krüger C,Hultberg A,Marcotte H,Hermans P,Bezemer S,Frenken LG,Hammarström L

更新日期：2006-10-01 00:00:00

abstract：:We describe a novel chromosome engineering technique for shuffling selected regions of chromosomes from two strains in Saccharomyces cerevisiae: The technique starts with the construction of MATa and MATalpha strains in which a particular chromosome is split at exactly the same site in both strains such that the split...

journal_title：Applied microbiology and biotechnology

authors： Sugiyama M,Yamamoto E,Mukai Y,Kaneko Y,Nishizawa M,Harashima S

更新日期：2006-10-01 00:00:00

abstract：:In this study, we purified and molecularly characterized a cold-active beta-galactosidase from Arthrobacter psychrolactophilus strain F2. The purified beta-galactosidase from strain F2 exhibited high activity at 0 degrees C, and its optimum temperature and pH were 10 degrees C and 8.0, respectively. It was possible to...

journal_title：Applied microbiology and biotechnology

authors： Nakagawa T,Fujimoto Y,Ikehata R,Miyaji T,Tomizuka N

更新日期：2006-10-01 00:00:00

abstract：:Respiration measurement in shake flasks is introduced as a new method to characterize the metabolic activity of microorganisms during and after stress exposure. The major advantage of the new method is the possibility to determine the metabolic activity independent of manual sampling without the necessity to change th...

journal_title：Applied microbiology and biotechnology

authors： Seletzky JM,Noack U,Fricke J,Hahn S,Büchs J

更新日期：2006-10-01 00:00:00

abstract：:A highly hydrophobic and non-toxic colloidal silica nanoparticle/polyvinyl butyral sol-gel composite membrane was prepared on a platinum wire electrode. With diphtheria-toxoid (D-Ag) as a model antigen and encapsulation of diphtheria antibody (D-Ab) in the composite architecture, this membrane could be used for reagen...

journal_title：Applied microbiology and biotechnology

authors： Wang FC,Yuan R,Chai YQ

更新日期：2006-10-01 00:00:00

abstract：:The nutritional medium requirement for biomass and triterpenoid production by Antrodia cinnamomea AC0623 strain was optimized. Box-Behnken was applied to optimize biomass and triterpenoid production. According to response surface methodology (RSM), the optimum concentrations of N-source were determined. The results in...

journal_title：Applied microbiology and biotechnology

authors： Chang CY,Lee CL,Pan TM

更新日期：2006-10-01 00:00:00

abstract：:Nutrition-based strategies to optimize xylose to ethanol conversion by Pichia stipitis were identified in growing and stationary-phase cultures provided with a defined medium varied in nitrogen, vitamin, purine/pyrimidine, and mineral content via full or partial factorial designs. It is surprising to note that station...

journal_title：Applied microbiology and biotechnology

authors： Slininger PJ,Dien BS,Gorsich SW,Liu ZL

更新日期：2006-10-01 00:00:00

abstract：:The human granulocyte macrophage colony-stimulating factor (GM-CSF) is a glycoprotein with important clinical applications for the treatment of neutropenia and aplastic anemia and reducing infections associated with bone marrow transplants. We evaluated the potential for using a potato virus X (PVX) viral vector syste...

journal_title：Applied microbiology and biotechnology

authors： Zhou F,Wang ML,Albert HH,Moore PH,Zhu YJ

更新日期：2006-10-01 00:00:00

abstract：:The manganese superoxide dismutase gene (sodM) is very highly expressed in Aspergillus oryzae. To elucidate the basis for this high-level expression, deletion analysis of the promoter was undertaken using beta-glucuronidase (GUS) as a reporter. Deletion of a 63-bp sequence from -200 to -138 in the 1,038-bp sodM promot...

journal_title：Applied microbiology and biotechnology

authors： Hisada H,Sano M,Ishida H,Hata Y,Abe Y,Machida M

更新日期：2006-10-01 00:00:00

abstract：:There is currently an increasing number of cytochrome P450 (CYP450) monooxygenase encoding genes becoming available from various genome-sequencing projects. These enzymes require association with cytochrome P450 reductase (CPR) to achieve optimal activities. In this study, the CYP53B1 gene, which encodes a benzoate pa...

journal_title：Applied microbiology and biotechnology

authors： Shiningavamwe A,Obiero G,Albertyn J,Nicaud JM,Smit M

更新日期：2006-09-01 00:00:00

abstract：:The rapid development of clean fuels for the future is a critically important global challenge for two main reasons. First, new fuels are needed to supplement and ultimately replace depleting oil reserves. Second, fuels capable of zero CO2 emissions are needed to slow the impact of global warming. This review summariz...

journal_title：Applied microbiology and biotechnology

authors： Rupprecht J,Hankamer B,Mussgnug JH,Ananyev G,Dismukes C,Kruse O

更新日期：2006-09-01 00:00:00

abstract：:Mycorrhizal fungi belonging to the genus Tuber produce, after the establishment of a productive interaction with a plant host, hypogeous fruitbodies of great economic value known as ''truffles''. This review summarizes the state of art on life cycle, genetic, and biotechnological investigations of Tuber spp. The ascoc...

journal_title：Applied microbiology and biotechnology

authors： Poma A,Limongi T,Pacioni G

更新日期：2006-09-01 00:00:00

abstract：:Eight Escherichia coli strains were studied in minimal medium with a continuous flow system using confocal microscopy. K12 wild-type strains ATCC 25404 and MG1655 formed the best biofilms ( approximately 43 microm thick, 21 to 34% surface coverage). JM109, DH5alpha, and MG1655 motA formed intermediate biofilms ( appro...

journal_title：Applied microbiology and biotechnology

authors： Wood TK,González Barrios AF,Herzberg M,Lee J

更新日期：2006-09-01 00:00:00

abstract：:Production of bacterial cellulose (BC) using sugar cane molasses (MO) with/without the presence of lignosulfonate (MOL) as a sole carbon source in a Hestrin-Schramm medium (HS) was investigated. Six strains of Acetobacter xylinum [American Type Culture Collection 10245 and Institute of Fermentation in Osaka (IFO) 1369...

journal_title：Applied microbiology and biotechnology

authors： Keshk S,Sameshima K

更新日期：2006-09-01 00:00:00

abstract：:Myxobacteria are Gram-negative soil microorganisms that prey on other microorganisms. Myxobacteria have significant potential for applications in biotechnology because of their extraordinary ability to produce natural products such as secondary metabolites. Myxobacteria also stand out as model organisms for the study ...

journal_title：Applied microbiology and biotechnology

authors： Ueki T,Inouye S

更新日期：2006-08-01 00:00:00

abstract：:The capability of lactic acid bacteria (LAB) to produce exopoly- and oligosaccharides was and is the subject of expanding research efforts. Due to their physicochemical properties and health-promoting potential, exopoly- and oligosaccharides from food-grade LAB can be used in the food and other industries and may have...

journal_title：Applied microbiology and biotechnology

authors： Korakli M,Vogel RF

更新日期：2006-08-01 00:00:00

abstract：:In this study, the kinetic behaviors between acetone and composite bead were investigated. The microbial growth rate decreased with increasing average inlet concentration and increased with increasing operation temperature at average inlet concentration ranging from 50 to 300 ppm and operation temperature ranging from...

journal_title：Applied microbiology and biotechnology

authors： Chan WC,Chang LY

更新日期：2006-08-01 00:00:00

abstract：:A novel system was developed for efficient transformation of the fungal biocontrol agent Beauveria bassiana. Competent blastospores were prepared and stored in LiAc- and glycerol-inclusive suspension at -76 degrees C for sequential use in transformation. The system was successfully applied to integrating phosphinothri...

journal_title：Applied microbiology and biotechnology

authors： Ying SH,Feng MG

更新日期：2006-08-01 00:00:00

abstract：:A new tool to provide an environmentally friendly way to deliver active proteins to the environment has been developed, based on the use of polyhydroxyalkanoate (PHA, bioplastic) granules. To illustrate this novel approach, a derived Cry1Ab insect-specific toxin protein was in vivo immobilized into PHA granules throug...

journal_title：Applied microbiology and biotechnology

authors： Moldes C,Farinós GP,de Eugenio LI,García P,García JL,Ortego F,Hernández-Crespo P,Castañera P,Prieto MA

更新日期：2006-08-01 00:00:00

abstract：:A soluble glucoside 3-dehydrogenase (G3DH) from Stenotrophomonas maltrophilia CCTCC M 204024, recently isolated from wheat soil in our laboratory, was purified to 37.4-fold with a yield of 24.7% and was estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis with a molecular mass of 66 kDa. 2,6-Dichloro...

journal_title：Applied microbiology and biotechnology

authors： Zhang JF,Zheng YG,Xue YP,Shen YC

更新日期：2006-08-01 00:00:00

abstract：:Gelatin zymography revealed the presence of proteolytic activity in conditioned medium (CM) from a serum-free, non-infected Spodoptera frugiperda, Sf9 insect cell culture. Two peptidase bands at about 49 and 39 kDa were detected and found to be proform and active form of the same enzyme. The 49-kDa form was visible on...

journal_title：Applied microbiology and biotechnology

authors： Lindskog E,Svensson I,Häggström L

更新日期：2006-07-01 00:00:00

abstract：:Analysis of gene expression data related to assimilation and biosynthesis of nitrogen-containing compounds amino acids, peptides, and nucleotides was used to monitor availability of these nutrients to Escherichia coli MG1655 growing on Luria-Bertani medium. The data indicate that free amino acids and nucleotides only ...

journal_title：Applied microbiology and biotechnology

authors： Baev MV,Baev D,Radek AJ,Campbell JW

更新日期：2006-07-01 00:00:00

abstract：:Mating tests, internal transcribed spacer (ITS) sequence analysis, intergenic spacer 1-restriction fragment length polymorphism (IGS1-RFLP), IGS1 sequence analysis, and IGS2-RFLP analysis were carried out on isolates of 17 morphologically different Pleurotus mushrooms collected on Ferula sinkiangensis. The isolates we...

journal_title：Applied microbiology and biotechnology

authors： Zhang JX,Huang CY,Ng TB,Wang HX

更新日期：2006-07-01 00:00:00

abstract：:Stable transformants of Chromobacterium violaceum were obtained by high-voltage electroporation with a 7-kilobase binary plasmid. The technique was reliable, reproducible, and simple, with efficiencies of 10(5) transformants/microg of plasmid DNA. The electrical conditions that resulted in the highest efficiencies wer...

journal_title：Applied microbiology and biotechnology

authors： Broetto L,Cecagno R,Sant'anna FH,Weber S,Schrank IS

更新日期：2006-07-01 00:00:00

abstract：:Real-time polymerase chain reaction (RT-PCR) was used to quantify seven species of lactic acid bacteria (LAB) in alfalfa silage prepared in the presence or absence of four commercial inoculants and in uninoculated corn stover harvested and stored under a variety of field conditions. Species-specific PCR primers were d...

journal_title：Applied microbiology and biotechnology

authors： Stevenson DM,Muck RE,Shinners KJ,Weimer PJ

更新日期：2006-07-01 00:00:00

abstract：:Aldolases are a specific group of lyases that catalyze the reversible stereoselective addition of a donor compound (nucleophile) onto an acceptor compound (electrophile). Whereas most aldolases are specific for their donor compound in the aldolization reaction, they often tolerate a wide range of aldehydes as acceptor...

journal_title：Applied microbiology and biotechnology

authors： Samland AK,Sprenger GA

更新日期：2006-07-01 00:00:00

abstract：:Pseudomonas strain CT14 was isolated from activated sludge. Strain CT14 contained a 55, 216 bp plasmid that was characterized by sequence analysis. The plasmid had a modular structure with 51 open reading frames (ORFs) that were distributed between two clearly demarcated domains. Domain I primarily contained genes for...

journal_title：Applied microbiology and biotechnology

authors： Bramucci M,Chen M,Nagarajan V

更新日期：2006-06-01 00:00:00

abstract：:Oils and fats are the most important renewable raw materials of the chemical industry. They make available fatty acids in such purity that they may be used for chemical conversions and for the synthesis of chemically pure compounds. Oleic acid (1) from "new sunflower," linoleic acid (2) from soybean, linolenic acid (3...

journal_title：Applied microbiology and biotechnology

authors： Metzger JO,Bornscheuer U

更新日期：2006-06-01 00:00:00

abstract：:Phosphate (P)-solubilizing microorganisms as a group form an important part of the microorganisms, which benefit plant growth and development. Growth promotion and increased uptake of phosphate are not the only mechanisms by which these microorganisms exert a positive effect on plants. Microbially mediated solubilizat...

journal_title：Applied microbiology and biotechnology

authors： Vassilev N,Vassileva M,Nikolaeva I

更新日期：2006-06-01 00:00:00